DNA Size Selection System 1 ChIP - Seq Library Prep Application Note 02

Low input DNA size selection on the Pippin Prep System using the Agilent 2100 Bioanalyzer with the Agilent High Sensitivity DNA kit

With the advent of next-generation sequencing (NGS), library preparation has become a much more streamlined process utilizing smaller amounts of input genomic DNA, thus allowing for more narrow fragmentation profi les. This Application Note demonstrates the usefulness of the Agilent 2100 Bioanalyzer and the Agilent High Sensitivity DNA kit in complementing the Pippin Prep automated size selecti...

TELP, a sensitive and versatile library construction method for next-generation sequencing

Next-generation sequencing has been widely used for the genome-wide profiling of histone modifications, transcription factor binding and gene expression through chromatin immunoprecipitated DNA sequencing (ChIP-seq) and cDNA sequencing (RNA-seq). Here, we describe a versatile library construction method that can be applied to both ChIP-seq and RNA-seq on the widely used Illumina platforms. Stan...

ILLUMINA SEQUENCING Whole-Genome Chromatin IP Sequencing (ChIP-Seq) Illumina ChIP-Seq combines chromatin immunoprecipitation (ChIP) with massively parallel DNA sequencing to identify binding sites of DNA-associated proteins. Illumina ChIP-Seq technology precisely and cost-effectively maps global binding sites for a protein of interest

INTRODUCTION Transcription factors and other chromatin-associated proteins are essential phenotype-influencing mechanisms. Determining how proteins interact with DNA to regulate gene expression is essential for fully understanding many biological processes and disease states. This epigenetic information is complimentary to genotype and expression analysis. Traditional methods have successfully ...

ILLUMINA SEQUENCING Whole-Genome Chromatin IP Sequencing (ChIP-Seq) Illumina ChIP-Seq combines chromatin immunoprecipitation (ChIP) with massively parallel DNA sequencing to identify binding sites of DNA-associated proteins. Illumina ChIP-Seq technology precisely and cost-effectively maps global binding sites for a protein of interest

INTRODUCTION Transcription factors and other chromatin-associated proteins are essential phenotype-influencing mechanisms. Determining how proteins interact with DNA to regulate gene expression is essential for fully understanding many biological processes and disease states. This epigenetic information is complimentary to genotype and expression analysis. Traditional methods have successfully ...

Library Prep Method Melds DNA Quantification and Size Selection into Single Step with Digital PCR | GenomeWeb